The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. 1 & 7. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. SpectraMax® M5 Multi-Mode Microplate Reader (cat. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. (Beijing, China) and a BCA protein assay kit from Beijing Solarbio Technology Co. For more sample throughput, the system can Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. Download PDF. spectramax m5 multi. Or, with the reader specifications noted from the Instrument Description section of your reader's. The system. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. TR-FRET assay principle. Measurements of fluorescence and fluorescence polarization were performed on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA) with the excitation and emission wavelengths 544 nm and 590 nm, respectively. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. Page 28 Chapter 5: Read Modes and Read Types 1. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. 5 12. Cavicide (Warehouse, Cat # 79300360). An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. Use similar settings for other Molecular Devices readers with fluorescence detection mode. The Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. The absorbance values at 450 nm were determined by the SpectraMax M5 microplate reader. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. 0 out of 5 $ 17,999 $ 16,995. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Introduction. Learn how to set up protocols, customize reports, export results, and more with this PDF document. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. 305 reference level saturation. Fluorescence from the Alamar blue® assay was detected by SpectraMax M5 microplate reader (Molecular Device Inc. AquaMax® 4000. ) by nonlinear regression using. 7°C and the bottom to 36. both cuvette-based and microplate reader applications on the same instrument. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. The dual monochromator optics allow. SpectraMax Lmicroplate luminometer. Microplate Readers Include the M3, M4, M5, and M5. The raw data from each individual experiment were normalized to vehicle-treated HK-2. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. Fluorescence Microplate Readers. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual. These lab workhorses are used in drug discovery, research, bioassay validation, and. The reader uses two scanning monochromators to determine the optimal excitation and emission settings for the spectral characteristics of. 0 and 0. TR-FRET assay principle. Detection of pro-inflammatory factorsThe SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Amino acids assay. flexibility of monochromator-based systems. SpectraMax® ABS. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 7 cells were co-transfected with 0. The advantage of using labeled probes is detection specificity. The K d was calculated with GraphPad Prism software (GraphPad Software Inc. ATG provides repair, maintenance, and contract services for laboratory research and bioprocess instruments. view more . Then, 150 μL of dimethyl sulfoxide was added to each well and shaken gently for 10 min. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. 87. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 7 1. Maximum signal with lowest background. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-,. begin reading microplates with seven mouse clicks. 0 μL) instead of the enzyme solution was used as the sample blank group. Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. After two more rinses with Hank’s solution, fluorescence intensity of the red/green ratio was determined on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at an excitation of 490 nm and emission of 530 nm (green fluorescent monomers) and 590 nm (red fluorescent aggregates) respectively. Detection of pro-inflammatory factorsThe SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. 2. Urea-induced unfolding of rA1AT-WT and the Q9N mutant was measured using a SpectraMax. Taurine ≥99 % was used as standard (Sigma-Aldrich ®,. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. The assay applies fluorescence polarization to quantify Fc-containing IgG. Ltd. SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. Selection of Water Constant in PathCheck settings. 6: bioNova científica, s. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. View the Molecular Devices website. For biological applications involving small samples, such as purificationThe guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. 0 OD for earlier models. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Introduction. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. Microplate Reader Documentation Version No. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence. Choose from a three- (M3), four- (M4), or0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Qualify the fluorescence performance of the SpectraMax . It measures the optical density (OD) of samples in a microplate or in a cuvette at aThe cells were then washed three times with PBS, and the fluorescent intensity of the cells was measured with a SpectraMax M5 microplate reader (Molecular Devices, USA) at an excitation wavelength of 488 nm and emission wavelength of 525 nm. When a feature or capability applies to only certain readers, this exception is noted. Bioz Stars score: 86/100, based on 1 PubMed citations. 40266 Email: drugdiscoverytech@lifetech. , USA). SpectraMax M5 Multi-Mode Microplate Reader. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Levels of immunoreactivity were normalized to. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. edu) to be added as a trained user in iLab. The guide also covers the software features, the specifications, and the accessories of the systems. ATP standard curves read on SpectraMax microplate readers. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. This application note describes how to. Read one sample or up to 384 inThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The. Microplate Readers include the M3, M4, M5, and M5e models. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 96-384. 49 2. Microplate Readers include the M3, M4, M5, and M5e models. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Sensitive fluorescent quantitation of DNA with the Quant-iT. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). The cuvette must be in place when you read the plate. Readings were taken using a UV–Vis Spectramax M5 ® microplate reader (Molecular Device, USA). SpectraMax® Aquamax. Italic and boldface type are used for emphasis. 1 Version 7. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. One vial of Thaw-and-Use TIGIT Effector Cells was thawed and resuspended in cell recovery. 05 3. Path Check Pathlength Measurement SpectraMax Spectrofluorometers. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideSpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. Filterless readers provide consistent results with steady temperature regulation. The cuvette must be in place when you read the plate. SpectraMax® M5. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. 5 87. In . B: Selection of Excitation Wavelength. com. Table of Contents. 4. Cables. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. • Tel. temperature and fluorescence was measured at Ex/Em 490/585 nm using a Molecular Devices SpectraMax M5 microplate reader. Absorbance was read on a Spectramax M5 Microplate reader at 450 nm. Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader. 1–0. Read More. The CellTiter-Glo assay from Promega uses luciferase. Molecular Devices SpectraMax M5 Multimode Microplate Reader. 00 USD. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Then, the fluorescence change rates (F 0 − F)/F 0 were calculated, and the selectivity histogram. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. The Multiskan Sky microplate reader is an absorbance plate reader. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Comprehensive software and validation Comprehensive data analysis and GxP solutions SoftMax. When the user applies a set temperature of 37°C, the top is heated to 37. With optimized reagents, option to operate the. Instrument settings are shown in Figure 3. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. The integration time suggested is 5-10 sec. B: Selection of Excitation Wavelength. 313 reference gain check fail. SpectraMax M5e Multi-Mode Microplate Reader. SpectraMax® ABS Plus. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Add to Cart. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. The absorbance value of each reaction solution was measured at 405 nm by a Molecular Device SpectraMax M5 microplate reader (Sunnyvale, CA, USA). FlexStation™ I. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Linear, Orbital Slow, and Orbital Fast. Example: [Drawer]. SpectraMax® ABS Plus. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. The absorbance was measured at 490 nm using a SpectraMax® M5 microplate reader (Molecular Devices, USA). Pick the best speed, sensitivity, and price for your needs. 3. Figure 1. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. 4. Documentation Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Lift up the lid over the chamber to insert or remove a cuvette. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. Compatible automation solutions for SpectraMax Readers. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. 5 Dual-luciferase reporter assay. l. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. STAKMAX. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Five preferred modes for diverse bioresearch applications. FlexStation™ I. Bottom-reading is usually recommended for cell-based assay with adherent cells. Molecular Devices Spectramax M5e Multi Mode Microplate Reader; Related products. Luminescence microplate readers or luminometers are used to detect luciferase-based reporter gene assays, chemiluminescent ELISAs, BRET, and many other assays. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected] or greater. edu) to be added as a trained user in iLab. Devices’ StakMax® Microplate Stacker in a matter of minutes and . The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Learn how you can utilize microplate readers to drive landmark discoveries. Plates were then shaken at room temperature for 10 min and the absorbance at 570/630 nm was measured by using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). When a feature or capability applies to only certain readers, this exception is noted. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Here the delta mP was 336, and Z’ factor was 0. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. SpectraMax microplate readers Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. Cuvette port functionality can also be controlled using SoftMax Pro Software. Fluorescent signals were measured using the SpectraMax/M5 fluorescence spectrophotometer plate reader (Molecular Devices Co. Anne T. RAW 264. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. flexibility of monochromator-based systems. As readout, fluorescence was measured at Ex/Em = 540/590 nm using a SpectraMax M5 microplate reader with SoftMax Pro v7 (Molecular devices) after 4–6 h of incubation in normal cell-culture medium. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. . Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). The following document is intended to demonstrate setup of this instrument. This reader's dual monochromators for. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Cuvette port functionality can also be controlled using SoftMax Pro Software. 0 out of 5 $ 17,999 $ 16,995. All reagents were brought to room temperature before use. Choose from a. For luminescence, the SpectraMax M5 Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Modethe introduction of the SPECTRAmax PLUS microplate spectrophotometer and its PathCheck feature, the ability to determine the pathlength in each well of a microplate and automatically normalize the absorbance value to a 1 cm pathlength. Softmax Pro GxP Software 6. Bookings are free, but you will need to use iLab to book. Wide range of applications. Shop now. The concentration of antibodies was determined by using a spectrophotometer from Puxi General Instrument Co. SpectraMax® iD5. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. PURPOSE. IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. AquaMax® 2000. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!The microplate readers in SpectraMax M5 series can also provide high-throughput capabilities with its automated microplate handling and storage systems. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. 05% Tween 20 using a Nunc ELISA plate washer. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. 7 cells were pretreated with SG (0, 25, 50, and 100 μg/mL). The following document is intended to demonstrate setup of this instrument. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Microplate Readers include the M3, M4, M5, and M5e models. #M5) SpectraMax® L Microplate Reader (cat. Detailed specifications of each model reader are reported in Section 7, “Specifications”. 4. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. No additional manuals are available. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit microplate reader. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. If you do not have an iLab account you must first make one: Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Note: In this user guide, SpectraMax ® refers to several SpectraMax . In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. No accessories are needed and SoftMax® Pro Software. 7°C (gradient heating design). Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Plate chamber design of the SpectraMax category 1 microplate readers. FlexStation™ III. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Choose from a three- (M3), four-. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. The day before assay, assay buffer was prepared as described in the product technical manual. book Page 7 Wednesday, July 7, 2010 2:09 PM. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 0 out of 5 $ 24,695. The TR-FRET signal was read on a TR-FRET microplate reader (SYNERGY H1, Biotek, Inc). For a higher . Introduction. 0 . Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini EM Reader, users never have to worry about not having the right set of filters. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell-based kinetic assays. iD5 i3x M5 Paradigm Delta mP 69 85 92 88 Average StDev (mP) 1. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. Protocol Name Version 6. • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. The absorbance was recorded using a SpectraMax M5 microplate reader (Molecular Devices). The assay window spanned four orders of magnitude. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. 16S rRNA gene sequencing . Note: In this user guide, all references to SpectraMax Multi-Mode. Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. Molecular Devices. SpectraMax iD5 Multi-Mode Microplate Reader. The CellTiter-Glo assay from Promega uses luciferase. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112. The SpectraMax® ABS Plus Microplate Reader can run . Turn on the microplate reader. The CDWIA was performed on each. It cannot be used for luminescence measurements. Most papers they cultured bacteria to OD600>0. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. Insert the cuvette into the chamber, making sure that the clear sides are to the left and right (facing the instrument). SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. SpectraMax Glo Steady-Luc Reporter Assay Kit. 9000-0756. When a FlexStation® 3 Multi-Mode Microplate Reader. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModePlate chamber design of the SpectraMax category 1 microplate readers. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. Fresh cecum content samples were collected for 16S rRNA sequencing by Shanghai Majorbio Bio-Pharm Technology Co. Sale price $16,495. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. The PBS solution (10. Add to Cart. Self Service Available. ) injection of 3 mLThe fluorescence intensity was detected by using a SpectraMax M5 microplate reader from Molecular Devices Co. IMAP FP Settings in SoftMax Pro M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. Then, after H 2 O 2 was added, the fluorescence was measured for each time period at 488 nm (excitation) and 525 nm (emission) wavelengths on a SpectraMax M5 microplate reader. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. View the Molecular Devices website. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Catalog Number: (89212-396) Supplier: Molecular Devices. Expanded wavelength coverage. SpectraTest Validation Plates form Molecular Devices such as ABS1 absorbance, FL1 fluorescence & LM1 luminescence validation plates are the valuable tools for verifying that absorbance, fluorescence, and luminescence-capable microplate readers are operating correctly for GLP and GMP laboratories. When the user applies a set temperature of 37°C, the top is heated to 37. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Spectramax M5 microplate reader: Molecular Devices: N/A:. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. FlexStation™ II. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. Our absorbance plate readers feature our PathCheck Sensor. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. A control experiment of the activity of the proposed enzyme/ZIF-8A particles without peptides against protease digestion was also performed. 67. MOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. Depending on which. Add to Cart. The assay window spanned four orders of magnitude. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. played in a grid format that corresponds to the wells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Western blotting assay. Microplate Readers are modular and . Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The PBS solution (10. TR-FRET assay. TR-FRET assay. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Figure 1.